Troubleshooting ENFINIA™ Linear DNA Migration on Agarose Gels

If your ENFINIA™ Linear DNA appears to run like a longer fragment on an agarose gel, this can be due to sequence adapters or DNA structure.

• ENFINIA™ Linear DNA contains:
  • 5’ adapter: 46 bp
  • 3’ adapter: 42 bp
• Total added length to your gene of interest: 88 bp
• Additional considerations: Secondary structures in the DNA can cause it to migrate more slowly in non-denaturing gels, making it appear larger than expected.

Recommendations:

• Account for the 88 bp of adapter sequence when comparing to size markers.
• For more accurate sizing, use denaturing gel conditions.
• If you continue to observe unusual migration patterns, contact customer.suppport@elegenbio.com and include agarose gel images along with your order details.