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Excising a Gene of Interest from ENFINIA™ Plasmid DNA

How to excise your gene of interest from Plasmid DNA

There are two ways to excise your gene of interest (GOI) from ENFINIA™ Plasmid DNA:

  1. Use restriction sites already present in the plasmid
  2. Add custom restriction sites to the DNA we synthesize

Steps:

  • Download the complete plasmid sequence (insert plus backbone):
    • Before order submission: On the Create Order page, next to Product Summary, click Export Analysis.
    • After order submission: On the My Orders page, in the Documents section, click Sequences.
  • For approach 1: Use DNA analysis software to identify unique restriction enzyme sites near your GOI.
  • For approach 2: Make sure your chosen restriction sites do not exist anywhere in the plasmid.