---
title: ENFINIA™ Linear DNA Applications
description: What to use Linear DNA for
---

[Skip to content](https://support.elegenbio.com/en/kb/enfinia-linear-dna-applications#main-content)

[Request Support](https://support.elegenbio.com/en/kb/kb-tickets/new?hsLang=en)

[![lockup-stroke-black-horz-sml-300](https://support.elegenbio.com/hs-fs/hubfs/lockup-stroke-black-horz-sml-300.png?width=300&height=67&name=lockup-stroke-black-horz-sml-300.png)](http://www.elegenbio.com/)

Open main navigation

Close main navigation

- [Request Support](https://support.elegenbio.com/en/kb/kb-tickets/new)
- [Elegen Ordering Portal](https://ecommerce.elegen.com/)

[Elegen Ordering Portal](https://ecommerce.elegen.com/)

 How can we help you?

- There are no suggestions because the search field is empty.

1. [Elegen Knowledgebase](https://support.elegenbio.com/en/kb?hsLang=en)
2. [Product Information](https://support.elegenbio.com/en/kb/product-information?hsLang=en)
3. [ENFINIA™ Linear DNA](https://support.elegenbio.com/en/kb/product-information?hsLang=en#enfinia-linear-dna)

November 20, 2025

# ENFINIA™ Linear DNA Applications

## What to use Linear DNA for

ENFINIA™ Linear DNA can be used directly in downstream applications including PCR, restriction enzyme digest, Gibson Assembly, and cell-free protein expression.

- For amplification and cloning recommendations, see this [user guide](https://elegenbio.com/amplification-and-cloning-guide-2/).
- For more details on Gibson Assembly reactions, see this [user guide](https://elegenbio.com/application-note-using-enfinia-linear-dna-in-gibson-assembly-reactions-2/).
- For more details on cell-free protein expression, see these two customer case studies: [Rapid Cell-Free Protein Expression with ENFINIA™ Linear](https://elegenbio.com/application-note-rapid-cell-free-protein-expression-with-enfinia-linear-dna-2/), [Accelerate Functional Screening with ENFINIA™ Linear DNA and GeneFrontier’s Cell-Free Protein Synthesis System](https://elegenbio.com/resources/case-studies/case-study-accelerate-functional-screening-with-enfinia-linear-dna-and-genefrontiers-cell-free-protein-synthesis-system/)

- [Getting Started](https://support.elegenbio.com/en/kb/getting-started?hsLang=en)
- [Troubleshooting](https://support.elegenbio.com/en/kb/troubleshooting?hsLang=en)
- [Product Information](https://support.elegenbio.com/en/kb/product-information?hsLang=en#main-content)

    - [ENFINIA™ Linear DNA](https://support.elegenbio.com/en/kb/product-information?hsLang=en#enfinia-linear-dna)
    - [ENFINIA™ Plasmid DNA](https://support.elegenbio.com/en/kb/product-information?hsLang=en#enfinia-plasmid-dna)
    - [ENFINIA™ IVT Ready DNA](https://support.elegenbio.com/en/kb/product-information?hsLang=en#enfinia-ivt-ready-dna)
    - [All ENFINIA™ DNA Products](https://support.elegenbio.com/en/kb/product-information?hsLang=en#all-enfinia-dna-products)
- [Shipping & Delivery](https://support.elegenbio.com/en/kb/shipping-delivery?hsLang=en)
- [Ordering](https://support.elegenbio.com/en/kb/ordering?hsLang=en)

[![Footer-Logo-\_-Tagline](https://support.elegenbio.com/hubfs/Footer-Logo-_-Tagline.svg "Footer-Logo-_-Tagline")](https://elegenbio.com)

[elegenbio.com](https://www.elegenbio.com)  
**©  2025 Elegen Corporation. All Rights Reserved.**

<https://www.linkedin.com/company/elegenbio/> <https://x.com/ElegenBio>

 GENERAL INQUIRIES  
[info@elegenbio.com](mailto:info@elegenbio.com)

## **Elegen Corp** 3565 Haven Ave, Suite 3 Menlo Park, CA 94025