Skip to content
  • There are no suggestions because the search field is empty.

ENFINIA™ IVT Ready DNA Quality Control

How IVT Ready DNA is quality controlled

As part of our QC process, we confirm the yield, sequence identity, and sequence quality of ENFINIA™ IVT Ready DNA.

  • Yield measurement: Performed using the DNA yield by Thermo Fisher Qubit 1X dsDNA High Sensitivity Assay Kit (Q33231).
  • Sequence identity: The customer provided sequence (excluding the poly(A) tail) is confirmed using long-read NGS.
  • Sequence quality: Synthesized DNA must meet internal QC thresholds to ensure proper poly(A) tailing and have minimal side product, as determined by long-read NGS.

Limitations: Current sequencing method cannot provide high-confidence measurements of homopolymers longer than 10 bp.

Poly(A) tail verification: Elegen has implemented additional QA steps to ensure poly(A) tail accuracy. Independent testing (see this white paper) demonstrates that ENFINIA™ IVT Ready DNA templates have accurate poly(A) tail length and low polydispersity compared with plasmid templates from a major supplier.