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ENFINIA™ IVT Ready DNA Length

What are the length limits for IVT Ready DNA

ENFINIA™ IVT Ready DNA sequences (excluding the poly(A) tail) can range in length from 1 kb to 5.5 kb.

For sequences shorter than 1 kb, a random nucleotide sequence can be added upstream of the promoter to extend the length to 1 kb. These additional nucleotides will not be transcribed. You can generate a random sequence using this tool, with the GC content set to 0.50.