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Definitions of ENFINIA™ DNA Sequence Acceptance Criteria

To evaluate whether a sequence is suitable for synthesis, we assess several sequence characteristics:

  • Overall GC content: The GC content across the entire sequence. 

  • 100 bp GC content: The minimum and maximum GC content in any 100 bp window along the sequence. 

  • Local GC variation: Within each 200 bp window, we evaluate smaller 20 bp sub-windows and measure how much their GC content deviates from the average GC content of that 200 bp window. 

  • Repeats: Regions of identical sequence that are ≥ 10 bp, either occurring contiguously (back-to-back) or repeated at different positions within the sequence.